Prince Harry reportedly spent the night of his 40th birthday with friends instead of spending it with Meghan.
The Duke of Sussex took off on a hiking trip to celebrate the landmark age milestone and made no mention of his wife in his birthday thanks message.
It comes after the couple made a series of separate plans, including Harrys solo trips to London and Lesotho in recent weeks.
The Duke has appeared at ten solo engagements in the last 15 days without his wife as he embarked on a quasi-royal tour across three countries, including New York, London and Lesotho.
Sources close to the Sussexs maintain that it was Meghan who booked the hiking trip for Harry and his pals.
Prince Harry reportedly spent the night of his 40th birthday with friends instead of spending it with Meghan
Prince Harry attends a Sentebale reception and panel discussion at The Saxon Hotel in Johannesburg on October 3
Harry greeting others at the Sentebale reception and panel discussion at The Saxon Hotel in South Africa
Harry taking part in the 14th Concordia Annual Summit in support of The Diana Award in New York last Monday
It has also been reported that Meghan bailed out on a charity event she was set to attend with Prince Harry because she was sick.
The last time Meghan was seen was the day before Harrys birthday and she was barely staying at their home in Montecito for periods of time earlier this year whilst he was said to be moping around by himself at a local cafe.
Last year, it was claimed that the pair spent extended spells apart but this was denied by sources close to them. It was said that, like any couple, they have disagreements but remained strong.
But Meghan has reportedly grown frustrated with Harrys court cases in the UK over his security entitlement.
It has also been reported that the couple have been in disagreement over how to raise their children, Archie, five, and Lilibet, three.
Harry did not want the children to be seen in public in case they were photographed but Meghan would apparently prefer to allow them to attend more local events, a source told The Sun.